Abstract: Objective To investigate the infection rate and type of human papillomavirus（HPV） in high-grade cervical squamous in-traepithelial lesions. Methods Types of HPV genes were detected using a gene-chip technique in 30 patients with CINⅡ-Ⅲ and 160 patients with cervical cancer.The relationship between HPV infection and cervical lesions was analyzed. Results Rates of HPV infec-tion in both groups were 90%，with 70.31%（19/27） and 81.94%（118/144） being single-type infections. HPV-58 and HPV-52 were the most frequent types among patients with CINⅡ-Ⅲ，while HPV-16 and HPV-18 were the most frequent types among patients with cervical cancer. HPV-16 was detected most frequently in both squamous cell carcinoma and adenocarcinoma. Conclusion HPV-16 and HPV-18 are the major HPV types associated with cervical cancer，while HPV-58 and HPV-52 are the major types associated with high-grade squamous intraepithelial lesions.

Key words: Human papillomavirus, High-grade squamous intraepithelial lesion, Cervical cancer